Effects of temperature, pH and NaCl content on in vitro putrescine and cadaverine production through the growth of Serratia marcescens CCM 303

Abstract

The aim of this study was to evaluate the combined effect of temperature (10, 20 and 37 degrees C), pH (4, 5, 6, 7 and 8), and NaCl content (0, 1, 3, 4, 5 and 6% w/v) on the growth and putrescine and cadaverine production of Serratia marcescens CCM 303 under model conditions. The decarboxylase activity of S. marcescens was monitored in broth after cultivation. The cultivation medium was enriched with selected amino acids (ornithine, arginine and lysine; 0.2% w/v each) serving as precursors of biogenic amines. Levels of putrescine and cadaverine in broth were analysed by high-performance liquid chromatography after pre-column derivatisation with o-phthalaldehyde reagent. S. marcescens produced higher amounts of putrescine (up to 2096.8mg L-1) compared to cadaverine content (up to 343.3mg L-1) in all cultivation media. The highest putrescine and cadaverine concentrations were reached during cultivation at 10-20 degrees C, pH 5-7 and NaCl content 1-3% w/v. On the other hand, the highest BAs production of individual cell (recalculated based on a cell; so called yield factor) was observed at 10 degrees C, pH 4 and salt concentration 3-5% w/v as a response to environmental stress.