Determination of ascorbic acid by electrochemical techniques and other methods

Abstract

Ascorbic acid (AA) is a water-soluble vitamin essential in the human nutrition, an antioxidant, a scavenger of free radicals in biological systems and a cofactor of several enzymes. For the precise detection of AA concentration in biological samples there is important the option of the determination method. To the most common and exact methods for AA evaluation belong HPLC techniques with UV and electrochemical detection, and electrochemical methods (EM). EM are simple, sensitive, and moderate methods. For AA detection different types of electrodes are suitable, lately there are used especially various chemically modified electrodes with applied polymer films. Popular and profitable for accurate AA determination there are electrochemical biosensors and voltammetry that is reputable due to the low detection limits of AA.