Nové aspekty symbiotické biodegradace polyvinyalkoholu

Abstract

Byla isolována nová kultura degradující polyvinylalkohol a identifikována jako Sphingomonas sp. Kultura vyžadovala k degradaci polyvinylalkoholu faktor PQQ a dále ještě jeden růstový faktor, který jí poskytovala symbiotická kultura Rhodococcus erythropolis. Výsledky ukázaly, že onen druhý růstový faktor může mít zásadní význam pro biodegradaci polymeru některými sfingomonádami.

A novel polyvinyl alcohol (PVA) degrading bacterium was isolated from activated sludge sampled during waste water treatment process and identified as Sphingomonas sp. Its PVA-oxidase activity and alcohol dehydrogenase activity for various low molecular weight secondary alcohols were detected. Both activities were associated with cells of the degrader and they were not extracellular. Under optimal conditions the isolate was able to degrade 500 mg of PVA per litre in two weeks. The strain required pyrroloquinoline quinone and another growth factor, the later supplied by co-isolated Rhodococcus erythropolis strain. The findings revealed a complex nature of the environmental PVA degradation and proved that other factor different from PQQ could be important for (in) biodegradation of the xenobiotic polymer with some sphingomonades.