Dual patterning of self-assembling spider silk protein nanofibrillar networks

Lamberger, Zan; Kocourková, Karolína; Minařík, Antonín; Humenik, Martin

dc.title	Dual patterning of self-assembling spider silk protein nanofibrillar networks	en
dc.contributor.author	Lamberger, Zan
dc.contributor.author	Kocourková, Karolína
dc.contributor.author	Minařík, Antonín
dc.contributor.author	Humenik, Martin
dc.relation.ispartof	Advanced Materials Interfaces
dc.identifier.issn	2196-7350 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2022-11
utb.relation.volume	9
dc.type	article
dc.language.iso	en
dc.publisher	Wiley
dc.identifier.doi	10.1002/admi.202201173
dc.relation.uri	https://doi.org/10.1002/admi.202201173
dc.relation.uri	https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/admi.202201173?download=true
dc.subject	nanofibrils	en
dc.subject	patterning	en
dc.subject	photolithography	en
dc.subject	proteins	en
dc.subject	self-assembly	en
dc.subject	silk	en
dc.description.abstract	Self-assembly of a recombinant spider silk protein into nanofibrillar networks in combination with photolithography is used to produce diversely functionalized micropattern. Amino-modified substrates coated with a positive tone photoresist are processed into 1 mu m deep arbitrarily shaped microwells, at the bottom of which spider silk proteins are covalently coupled to the deprotected aminated surface. The protein layer serves to seed the self-assembly of nanofibrils from the same protein in the microwells, forming immobilized few nanometers thin networks after the stripping of the photoresist. The nanofibrous micropattern can be functionalized by employing fluorescently modified spider silk variants during the self-assembly or by later covalent modification with nucleic acids. By repeating the photolithography and fibril assembly procedures, two functionally different and spatially defined pattern are created.	en
utb.faculty	Faculty of Technology
utb.faculty	University Institute
dc.identifier.uri	http://hdl.handle.net/10563/1011134
utb.identifier.obdid	43884006
utb.identifier.scopus	2-s2.0-85137825437
utb.identifier.wok	000852788400001
utb.source	J-wok
dc.date.accessioned	2022-09-20T08:07:44Z
dc.date.available	2022-09-20T08:07:44Z
dc.description.sponsorship	Bavarian-Czech Academic Agency (Bayerisch-Tschechische Hochschulagentur) BTHA Grant [JC-2019-21]; Czech Science Foundation [22-33307S]; TBU Grant [IGA/FT/2022/009]; Projekt DEAL
dc.description.sponsorship	JC‐2019‐21; Tomas Bata University in Zlin, TBU: IGA/FT/2022/009; Grantová Agentura České Republiky, GA ČR: 22–33307S
dc.rights	Attribution 4.0 International
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.rights.access	openAccess
utb.ou	Department of Physics and Materials Engineering
utb.ou	Centre of Polymer Systems
utb.contributor.internalauthor	Kocourková, Karolína
utb.contributor.internalauthor	Minařík, Antonín
utb.fulltext.affiliation	Zan Lamberger, Karolína Kocourková, Antonín Minařík, and Martin Humenik* Z. Lamberger,[+] M. Humenik Department of Biomaterials Faculty of Engineering Science Universität Bayreuth Prof.-Rüdiger-Bormann.Str. 1, 95447 Bayreuth, Germany E-mail: [email protected] K. Kocourková, A. Minařík Department of Physics and Materials Engineering Faculty of Technology Tomas Bata University in Zlín Vavrečkova 275, Zlín 760 01, Czech Republic K. Kocourková, A. Minařík Centre of Polymer Systems Tomas Bata University in Zlín Třída Tomáše Bati 5678, Zlín 76001, Czech Republic [+]Present addresses: Department of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany
utb.fulltext.dates	Received: May 26, 2022 Revised: August 16, 2022 First published: 12 September 2022
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utb.fulltext.sponsorship	This work was financially supported by the Bavarian-Czech Academic Agency (Bayerisch-Tschechische Hochschulagentur) BTHA Grant No. JC-2019-21 and Czech Science Foundation (Project No. 22–33307S). The work of author K.K. was financially supported by TBU Grant No. IGA/FT/2022/009. The authors thank Prof. Thomas Scheibel, Chairholder of Department Biomaterials, University Bayreuth, for providing the facility to conduct this research. Open access funding enabled and organized by Projekt DEAL.
utb.wos.affiliation	[Lamberger, Zan; Humenik, Martin] Univ Bayreuth, Fac Engn Sci, Dept Biomat, Prof Rudiger Bormann Str 1, D-95447 Bayreuth, Germany; [Kocourkova, Karolina; Minarik, Antonin] Tomas Bata Univ Zlin, Fac Technol, Dept Phys & Mat Engn, Vavreckova 275, Zlin 76001, Czech Republic; [Kocourkova, Karolina; Minarik, Antonin] Tomas Bata Univ Zlin, Ctr Polymer Syst, Trida Tomase Bati 5678, Zlin 76001, Czech Republic
utb.scopus.affiliation	Department of Biomaterials, Faculty of Engineering Science, Universität Bayreuth, Prof.-Rüdiger-Bormann.Str. 1, Bayreuth, 95447, Germany; Department of Physics and Materials Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, Zlín, 760 01, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlín, Třída Tomáše Bati 5678, Zlín, 76001, Czech Republic; [+]Present addresses: Department of Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, Würzburg, 97070, Germany
utb.fulltext.projects	BTHA JC-2019-21
utb.fulltext.projects	GACR 22–33307S
utb.fulltext.projects	IGA/FT/2022/009
utb.fulltext.faculty	Faculty of Technology
utb.fulltext.faculty	University Institute
utb.fulltext.ou	Department of Physics and Materials Engineering
utb.fulltext.ou	Centre of Polymer Systems
utb.identifier.jel	-