Preparation of collagen-elastin hydrolysates from cattle tendons and studying properties of hydrolysates

Abstract

The contribution characterises properties of collagen-elastin hydrolysates (content of proteins, collagen, elastin, ash and molecular weights) prepared by a 3-stage procedure from cattle tendons. Depending on conditions in 1st and 3rd stage of the process, hydrolysates may be prepared possessing molecular weights of 900-8,000 Da. Lowest ash content 3.2 % (w/w) was found with hydrolysates prepared from tendons processed in alkaline environment. Dialysis or ion exchange treatment allows to further reduce ash content. The ratio of collagen and elastin contents in hydrolysates differs in accordance with mode of processing - from 1.34 times collagen excess, through a balanced share of both proteins up to 1.21 times excess of elastin. In dependence on procedure conditions (tendon pre-processing at pH levels 4-11, extraction at 60-100 degrees C for duration 2-14 min), hydrolysates of collagen-elastin type may be prepared, displaying required properties and possessing a milky colour. Our hydrolysates are equal in quality to commercially produced collagen hydrolysates for cosmetic or food applications.