Phenolic profile of edible honeysuckle berries (genus Lonicera) and their biological effects

Juríková, Tünde; Rop, Otakar; Mlček, Jiří; Sochor, Jiří; Balla, Štefan; Szekeres, Ladislav; Hegedüsová, Alžběta; Hubálek, Jaromír; Adam, Vojtěch; Kizek, René

dc.title	Phenolic profile of edible honeysuckle berries (genus Lonicera) and their biological effects	en
dc.contributor.author	Juríková, Tünde
dc.contributor.author	Rop, Otakar
dc.contributor.author	Mlček, Jiří
dc.contributor.author	Sochor, Jiří
dc.contributor.author	Balla, Štefan
dc.contributor.author	Szekeres, Ladislav
dc.contributor.author	Hegedüsová, Alžběta
dc.contributor.author	Hubálek, Jaromír
dc.contributor.author	Adam, Vojtěch
dc.contributor.author	Kizek, René
dc.relation.ispartof	Molecules
dc.identifier.issn	1420-3049 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2012
utb.relation.volume	17
utb.relation.issue	1
dc.citation.spage	61
dc.citation.epage	79
dc.type	article
dc.language.iso	en
dc.publisher	MDPI AG	en
dc.identifier.doi	10.3390/molecules17010061
dc.relation.uri	http://www.mdpi.com/1420-3049/17/1/61/
dc.subject	edible honeysuckle	en
dc.subject	polyphenolic compounds	en
dc.subject	anthocyanins	en
dc.subject	antioxidant activity	en
dc.subject	biological effect	en
dc.description.abstract	The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed. The major classes of phenolic compounds in the blue berried honeysuckle are flavonols (quercetin, rutin, quercitrin) and flavanes (proanthocyanidins, catechins) and anthocyanins. Cyanidin-3-glucoside and cyanidin-3-rutinoside are considered as major anthocyanidins in edible honeysuckle berries. Such a high level of antioxidant activity in the berries of different species of the genus Lonicera is especially due to the high level of polyphenolic compounds, especially anthocyanins. These berries seem to be prospective sources of health-supporting phytochemicals that exhibit beneficial anti-adherence and chemoprotective activities, thus they may provide protection against a number of chronic conditions, e.g., cancer, diabetes mellitus, tumour growth or cardiovascular and neurodegenerative diseases.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1002730
utb.identifier.rivid	RIV/70883521:28110/12:43867351!RIV13-MSM-28110___
utb.identifier.obdid	43867611
utb.identifier.scopus	2-s2.0-84856181860
utb.identifier.wok	000299535700005
utb.identifier.coden	MOLEF
utb.source	j-wok
dc.date.accessioned	2012-02-29T13:04:46Z
dc.date.available	2012-02-29T13:04:46Z
dc.rights	Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.access	openAccess
utb.contributor.internalauthor	Rop, Otakar
utb.contributor.internalauthor	Mlček, Jiří
utb.fulltext.affiliation	Tunde Jurikova 1, Otakar Rop 2, Jiri Mlcek 2, Jiri Sochor 3,4, Stefan Balla 1, Ladislav Szekeres 1, Alzbeta Hegedusova 5, Jaromir Hubalek 3,6, Vojtech Adam 3,4 and Rene Kizek 3,4,* 1 Faculty of Central European Studies, Institute of Natural and Informatics Sciences, Constantine the Philosopher University in Nitra, Nabrezie mladeze 91, SK-949 76 Nitra, Slovakia 2 Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlin, Namesti T. G. Masaryka 275, CZ-762 72 Zlin, Czech Republic 3 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 4 Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic 5 Department of Chemistry, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Tr. A. Hlinku 1, SK-949 76 Nitra, Slovakia 6 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, CZ-616 00 Brno, Czech Republic * Author to whom correspondence should be addressed; E-Mail: [email protected].
utb.fulltext.dates	Received: 18 November 2011 in revised form: 14 December 2011 Accepted: 19 December 2011 Published: 22 December 2011
utb.fulltext.references	1. Kühnau, J. The flavonoids: A class of semi-essential food cxomponents: Their role in human nutrition. In World Review of Nutrition and Dietetics, 1st ed.; Karger: Basel, Switzerland, 1976; Volume 24, pp. 117-191. 2. Pheron, N.T.; Brumaghim, J.L. A review of the antioxidant mechanism of polyphenol compounds related to iron binding. Cell Biochem. Biophys. 2009, 53, 75-100. 3. Velisek, J. Chemie Potravin, 1st ed.; OSSIS: Tabor, Czech Republic, 1999; pp. 727-747. 4. Vattem, D.A.; Ghaedian, R.; Shetty, K. Enhancing health benefits of berries through phenolic antioxidant enrichment: Focus on cranberry. Asia Pac. J. Clin. Nutr. 2005, 14, 120-130. 5. Seeram, N.P.; Heber, D. Impact of berry phytochemicals on human health: Effects beyond antioxidation. In Lipid Oxidation and Antioxidants: Chemistry, Methodologies and Health Effects; Ho, C.T., Shahidi, F.S., Eds.; Oxford University Press: New York, NY, USA, 2006; ACS Symposium Series 956, pp. 240-255. 6. Rop, O.; Jurikova, T.; Mlcek J.; Kramarova, D.; Zultsetseg, S. Antioxidant activity and selected nutritional values of plums (Prunus domestica L.) typical of the White Carpathian Mountains. Sci. Hortic. 2009, 122, 545-549. 7. Seeram, N.P. Berry Fruits for Cancer Prevention: Current Status and Future Prospects. J. Agric. Food Chem. 2008, 56, 630-635. 8. Gruia, M.I.; Oprea, E.; Gruia, I.; Negoita, V.; Farcasanu, I.C. The antioxidant response induced by Lonicera caerulaea berry extracts in animals bearing experimental solid tumors. Molecules 2008, 13, 1195-1206. 9. Baranec, T.; Polacikova, M.; Kostal, J. Systematicka Botanika, 1st ed.; SPU: Nitra, Slovak Republic, 1988; pp. 206-210. 10. Baranec, T.; Polacikova, M.; Kostal, J. Systematicka Botanika, 2nd ed.; SPU: Nitra, Slovak, 1998; pp. 206-208. 11. Plekhanova, M.N.; Streltsyna, S.A. Fruit Chemical Composition of Lonicera subsect. Caerulea (Caprifoliaceae species), Genetic Resourses in Russia and Neighbouring Countries; Estonian Agricultural University-Forest Research Institut: Tartu, Estonia, 1998; pp. 143-146. 12. Sokolov, P.D. Plant Resorces of the USSR: Flowering Plants, their Chemical Composition and Utilization. Caprifoliaceae—Plantaginaceae Families, 1st ed.; Nauka: Leningrad, Soviet Union, 1988; pp. 77-78. 13. Rop, O.; Reznicek, V.; Mlcek, J.; Jurikova, T.; Balik, J.; Sochor, J.; Kramarova, D. Antioxidant and radical oxygen species scavenging activities of 12 cultivars of blue honeysuckle fruit. Hort. Sci. 2011, 38, 63-70. 14. Thompson, M.M.; Barney, D.L. Evaluation and Breeding of Haskap in North America. J. Am. Pomol. Soc. 2007, 61, 25-33. 15. Chaovanalikit, A.; Thompson, M.M.; Wrolstad, R.E. Characterization and quantification of anthocyanins and polyphenolics in bluehHoneysuckle (Lonicera caerulea L.). J. Agric. Food Chem. 2004, 52, 848-852. 16. Pokorna, T.; Matuskovic, J. The study of irrigation influence on nutritional value of Lonicera kamtschatica—Cultivar Gerda 25 and Lonicera edulis berries under the Nitra conditions during 2001-2003. Hort. Sci. 2007, 34, 11-16. 17. Plekhanova, M.N. Žimolosť sinnaja v sadu i pitomke, 1st ed.; Vserossijskij naučnoissledovateľskij institut rastenjevodstva I.V.Vavilova: Sant Peterburg, Russia, 1998; pp. 65-69. 18. Vereshchagin, A.L.; Anikina, E.V.; Syrchina, A.I.; Lapin, M.F.; Azin, A.; Semenov, A.A. Chemical investigation of the bitter substances of the fruit of Lonicera caerulea. Chem. Nat. Compd. 1989, 25, 289-292. 19. Plekhanova, M.N. Blue honeysuckle (Lonicera caerulea L.)—A new commercial berry crop for temperate climate: Genetic resources and breeding. Acta Hortic. 2000, 538, 215-220. 20. Fan-Chiang, H.J.; Wrolstad, R.E. Anthocyanin pigment composition of blacberries. J. Food Chem. 2005, 70, 197-202. 21. Jurikova, T.; Matuškovič, J.; Jurik, I.; Moravčik, J. Agrobiological Aspects of Cultivation of the Edible Honeysuckle, Nutrition Value and Content of Anthocyanins in Berries, 1st ed.; SPU: Nitra, Slovak, 2010; pp. 133-135. 22. Jurikova, T.; Paulovicsova, B.; Baloghova, M.; Matuskovic, J.; Gazdik, Z.: Therapeutic properties of edible honeysuckles and their testing on laboratory animals. Slovak Vet. J. 2009, 10, 23-24. 23. Deineka, V.I.; Sorokopudov, V.N.; Deineka, L.A.; Shaposhnik, E.I.; Koltsov, S.V. Anthocyans from fruit of some plants of the Caprifoliaceae family. Chem. Nat. Compd. 2005, 41, 162-164. 24. Hummer, K.E. Blue honeysuckle in New Fruit and Nut Cultivars. HortScience 2006, 41, 1107-1109. 25. Hummer, K.E. Blue honeysuckle: A new berry crop for North America. J. Am. Pomol. Soc. 2006, 60, 3-8. 26. Plekhanova, M.N. Aktinidija, limonik, žimolosť, 2nd ed.; Agromizdat: Leningrad, Russia, 1990; 85-87. 27. Hedin, P.A.; Philips, V.A. Volatile constituents from honeysuckle aphids and the honeysuckle, Lonicera spp., search for assembling pheromones. J. Agric. Food Chem. 1991, 39, 1304-1306. 28. Bakowska, A.; Kucharska, A.Z.; Oszmianski, J. The effect of heating, UV-irradiation, and storage on stability of the anthocyanin—Polyphenol copigment complex, Food Chem. 2003, 81, 349-355. 29. Plekhanova, M.N.; Streltsyna, S.A.; Rostova, N.S. Phenolic compounds in berries of Lonicera subsect. Caerulea species. Plant Res. 1993, 29, 16-25. 30. Trajkovski, V.; Hjalmarsson, V. The value of national fruit gene banks. Žemés Úkio Mokslai 2007, 14, 28-32. 31. Plekhanova, M.N. Osobenosti fenologii žimolosti so sjedobnymi plodmi v Leningradskej oblasti, Trudy po priklodnoj botanike, genetike i selekcii, 2nd ed.; Agromizdat: Leningrad, Soviet Union, 1978; pp. 104-114. 32. Herrmann, K. Occurrence and content of hydroxycinnamic and hydroxybenzoic acid compounds in foods. Crit. Rev. Food Sci. 1989, 28, 315-347. 33. Clifford, M.N. Chlorogenic acids and other cinnamates—Nature, occurrence, dietary burden, absorption and metabolism. J. Sci. Food Agr. 2000, 80, 1033-1043. 34. Donovan, J.L.; Meyer, A.S.; Waterhouse, A.L. Phenolic Composition and Antioxidant Activity of Prunes and Prune Juice (Prunus domestica). J. Agric. Food Chem. 1998, 46, 1247-1252. 35. Belitz, H.D.; Grosch, W. Food Chemistry, 4th ed.; Springer Verlag: Berlin, Germany, 1999; pp. 900-992. 36. Rop, O.; Jurikova, T.; Sochor, J.; Mlcek, J.; Kramarova, D. Antioxidant capacity, scavenging radical activity and selected chemical composition of native apple cultivars from Central Europe. J. Food Q. 2011, 34, 187-194. 37. Moyer, R.A.; Hummer, K.E.; Finn, C.E.; Frei, B.; Wrolstad R.E. Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, rubus, and ribes. J. Agr. Food Chem. 2002, 50, 519-525. 38. Zhu, X.; Zhang, H.; Lo, R. Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J. Agric. Food Chem. 2004, 52, 7272-7278. 39. Zadernowski, R.; Naczk, M.; Nesterowicz, J. Phenolic acid profiles in some small berries. J. Agr. Food Chem. 2005, 53, 2118-2124. 40. Jusev, A.A.; Plekhanova, M.N. Samyje lučšije, 1st ed.; Vserossijskij naučno-issledovateľskij institut rastenijevodstva I. Vavilova: Sankt Peterburg, Russia, 1997; pp 120-122. 41. Orincak, J.; Matuskovic, J.; Jurcak, S. Possibilities of Species Lonicera caerulea in Utilization of the Secondary Metabolism in Food and Pharmaceutical Processing, 1st ed.; SPU: Nitra, Slovak, 2003; pp. 210-219. 42. Lee, J.; Durst, R.; Wrolstad, R.E. Impact of juice processing on blueberry anthocyanins and polyphenolics: Comparison of two treatments. J. Food. Sci. 2002, 67, 1660-1667. 43. Kahkonen, M.; Hopla, A.; Heinonen, M. Berry phenolics and their antioxidant activity. J. Agr. Food Chem. 2001, 49, 4076-4082. 44. Manach, C.; Scalbert, A.; Morand, Ch.; Rémésy, Ch.; Juménez, L. Polyphenols: Food resources and bioavaibility. Am. J. Clinic. Nutr. 2004, 74, 727-747. 45. Gazdik, Z.; Reznicek, V.; Adam, V.; Zitka, O.; Jurikova, T.; Krska, B.; Matuskovic, J.; Plsek, J.; Saloun, J.; Horna, A.; Kizek, R. Use of liquid chromatography with electrochemical detection for the determination of antioxidants in less common fruits. Molecules 2008, 13, 2823-2836. 46. Harborne, J.B.; Williams, C.A. Advances in flavonoid research since 1992. Biofactors 2000, 6, 481-504. 47. Petrova, V.P. Biochimija dikorastuščich plodovo—jagodnych rastenij, 1st ed.; Golovnoe izdateľstvo objedenija: Kijev, Ukraine, 1986; pp. 260-266. 48. Bors, W.; Michael, C.; Stettmaier, K. Antioxidant effect of flavonoids. Biofactors 1997, 6, 399-402. 49. Yang, B.; Kotani, A.; Arai, K.; Kusu, F. Estimation of the antioxidant activities of flavonoids from their oxidation potentials. Anal. Sci. 2001, 17, 599-604. 50. Sapiro, D.K.; Anichimovskaja, L.V.; Parižnaja, T.I. Biochimičeskij sostav sjedobnych plodov Lonicera L. introdukovaných v Bellorusije. Rastiteľnyje Res. 1981, 4, 565-568. 51. Chaovanalikit, A.; Wrolstad, R.E. Anthocyanin and polyphenolic composition of fresh and processed cherries. J. Food Sci. 2004, 69, 69-78. 52. Andersen, M.; Jordheim, M. The Anthocyanins. Chemistry, Biochemistry and Applications, 4th ed.; CRC Press: Boca Raton, FL, USA, 2007; pp. 471-553. 53. Jordheim, M.; Giske, N.H.; Andersen, O.M. Anthocyanins in Caprifoliaceae. Biochem. Syst. Ecol. 2007, 35, 153-159. 54. Gazdik, Z.; Krska, B.; Adam, V.; Saloun, J.; Jurikova, T.; Reznicek, V.; Horna, A.; Kizek, R. Electrochemical determination of antioxidant potential of some less common fruit species. Sensors 2008, 8, 7564-7570. 55. Terahara, N.; Tomoaki, S.; Tsukui, A. Anthocyanins from berries of Haskaap, Lonicera caeruleaa L. J. Home Econ. Jpn. 1993, 44, 197-201. 56. Mariassyova, M.; Silhar, S.; Kovac, M. New sources of anthocyans, Finland: Agri-Food Quality Managment of Fruits and Vegetables, 1st ed.; Royal Society of Chemistry-Agricultural Research centre of Finland: Helsinki, Finland, 1999; pp. 311-313. 57. Caganova, I. The genus Lonicera L.—A Valuable Genus Resource for New Crop, Plant Genetic Resources, 1st ed.; SPU: Nitra, Slovak, 1993; pp. 99-102. 58. Bakowska, A.M.; Marianchuk, M.; Kolodziejczyk, P. Survey of bioactive components in Western Canadian berries. Can. J. Physiol. Pharmacol. 2007, 85, 1139-1152. 59. Rop, O.; Mlcek, J.; Jurikova, T.; Valsikova, M.; Sochor, J.; Reznicek, V.; Kramarova, D. Phenolic content, antioxidant capacity, radical oxygen species scavenging and lipid peroxidation inhibiting activities of extracts of five black chokeberry (Aronia melanocarpa (Michx.) Elliot) cultivars. J. Med. Plants Res. 2010, 4, 2431-2437. 60. Turianica, I.; Jurikova, T.; Paulovicsova, B.; Baloghova, M.; Matuskovic, J. Non-traditional fruit species as a important source of natural colouring matters. Vistnik stomatologii 2008, 16, 41-42. 61. Paulovicsova, B.; Turianica, I.; Jurikova, T.; Baloghova, M.; Matuskovic, J. Antioxidant properties of selected less common fruit species. Lucrari Stiifice Zootehnie Biotech. 2009, 42, 608-613. 62. Baloghova, M.; Paulovicsova, B.; Turianica, I. Biological characteristics of selected berries. Acta Hort. Regio. 2009, 12, 49-52. 63. Jurikova, T.; Saloun, J.; Beklova, M.; Frolkova, P.; Kizek, R.: Interdisciplinary Dialogue on Potential Health Benefits from Blue Honeysuckle (Lonicera edulis), (Lonicera kamtschatica) and their Hybrids with Respect to Combinatorial Investigation of Antimicrobial Effects and Antioxidant Properties, 1st ed.; SPU: Nitra, Slovak, 2009; pp. 151-159. 64. Bryksin, D.M. Ocenka plodov sortoobrazcov žimolosti k zamarozke, Netradicionaľnije prirodnyje resurcy, inovacionnyje technologii I produkty, zbornik naučnych trudov, 1st ed.; Bypusk: Moscow, Russia, 2007; pp. 206-210. 65. Mazza, G.; Broillard, R. The mechanism of co-pigmentation of anthocyanins in food products. Food Chem. 1990, 25, 207-225. 66. Davies, A.J.; Mazza, G. Copigmentation of simple and acylated anthocyanins with colorless phenolic compounds. J. Agric. Food Chem. 1993, 41, 716-720. 67. Koh, M.S; Hwang, J.S.; Moon, A. Lycopene inhibits proliferation, invasion and migration of human breast cancer cells. Biomol. Ther. 2010, 18, 92-98. 68. Fu, L.S.; Okamoto, H.; Kataoka, T.; Shibata, Y. Color based classification for berries of Japanese Blue Honeysuckle. Int. J. Food Eng. 2011, 7, 131-135. 69. Wang, Q. Neuroprotective mechanism of curcumin against cerebral ischemia – induced neuronal apoptosis and behavioral deficits. J. Neurosci. Res. 2005, 82, 138-148. 70. Gabrielska, J.; Oszmianski, J.; Komorowska, M. Anthocyanin extracts with antioxidant and radical scavening efect, Zeitschrift fur naturforschung. Biosciences 1999, 54, 319-324. 71. Thompson, M.N.; Chaovanalikit, A. Preliminary observations on adaptation and nutraceutical values of blue honeysuckle (Lonicera caerulea) in Oregon, USA. Acta Hortic. 2003, 626, 65-72. 72. Siriwoharn, T.; Wrolstad, R.E. Polyphenolic compounds of marion and evergreen blackberries. J. Food Sci. 2004, 69, 233-240. 73. Litescu, S.C.; Oprea, E.; Diaconu, M.; Radu, G.L. A rapid determination of radical scavenger properties of plant extracts using electrochemical approach. Rev. Roum. Chim. 2011, 56, 25-26. 74. I. Palikova, I.; Heinrich, J.; Bednar, P.; Marhol, P.; Kren, V.; Cvak, L.; Valentova, K.; Ruzicka, F.; Hola, V.; Kolar, M.; Simanek, V.; Ulrichova, J. Constituents and antimicrobial properties of blue honeysuckle: A novel source for phenolic antioxidants. J. Agric. Food Chem. 2008, 56, 11883-11889. 75. Caganova, I. Rod Lonicera ako ovocny druh. Zahradnictvi 1994, 40, 103-108. 76. Palikova, I.; Valentova, K.; Oborna, I.; Ulrichova, J. Protectivity of blue honeysuckle extract against oxidative human endothelial cells and rat hepatocyte damage. J. Agric. Food Chem. 2009, 57, 6584-6589. 77. Duthie, S.J.; Jenkinson, A.M.; Crozier, A.; Mullen, W.; Pirie, L.; Kyle, J.; Zap, L.S.; Christen, P.; Duthie, G.G. The effects of cranberry juice consumption on antioxidant status and biomarkers relating to heart disease and cancer in healthy human volunteers. Eur. J. Nutr. 2006, 45, 113-122. 78. Zheng, J.; Wang, Q.; Kim, M.; Lian, J.; Ng L.S. Experimental study of antifatigue effects of Lonicera edulis. J. Med. Sci. Yanbian Uni. 2001, 5, 16-17. 79. Zheng, J.; Wang, Q.; Kim, M.; Lee, W.; Hong, S. Histochemical study of protective effects of the Lonicera edulis for mice liver damaged by carbon tetrachloride. J. Med. Sci. Yanbian Uni. 2001, 5, 18-19. 80. Guang, J.; Yue Y.; Hong S. Therapeutic effects of Lonicera edulis alcoholic extract on adjusting blood lipid in rats. J. Med. Sci. Yanbian Uni. 2004, 8, 109-111. 81. Wang, Q. Neuroprotective mechanism of curcumin against cerebral ischemia—Induced neuronal apoptosis and behavioral deficits. J. Neurosci. Res. 2005, 82, 138-148. 82. Wang, Q.; Jin, Z.; Li, X.W.; Jin, M.S. Effect of Lonicera edulis on serum glutamic oxaloacetic transaminase enzyme in mice liver damaged by carbon tetrachloride. J. Med. Sci. Yanbian Uni. 2001, 5, 191-192. 83. Son, M.J.; Moon, T.C.; Lee, E.K.; Son, K.H.; Kim, H.P.; Kang, S.S.; Son, J.K.; Lee, S.H.; Chang, H.W. Naturally occurring biflavonoid, ochnaflavone, inhibits cyclooxygenases-2 and 5- lipoxygenase in mouse bone marrow-derived mast cells. Arch. Pharm. Res. 2006, 4, 282-286. 84. Zdarilova, A.; Svobodova, A.R.; Chytilova K.; Simanek, V.; Ulrichova, J. Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblast. Food Chem. Toxicol. 2010, 48, 1555-1561. 85. Svobodova, A.; Zdarilova, A.; Vostalova, J. Lonicera caerulea and Vaccinium myrtillus fruit polyphenols protect HaCaT keratinocytes against UVB-induced phototoxic stress and DNA damage. J. Dermatol. Sci. 2009, 56, 196-204. 86. Rop, O.; Reznicek, V.; Valsikova, M.; Jurikova, T.; Mlcek, J; Kramarova, D. Antioxidant Properties of European Cranberrybush Fruit (Viburnum opulus var. edule). Molecules 2010, 15, 4467-4477. 87. Zitka, O.; Sochor, J.; Rop, O.; Skalickova, S.; Sobrova, P.; Zehnalek, J.; Beklova, M.; Krska, B.; Adam, V.; Kizek, R. Comparison of various easy-to-use procedures for extraction of phenols from apricot fruits. Molecules 2011, 16, 2914-2936. 88. Sochor, J.; Zitka, O.; Skutkova, H.; Pavlik, D.; Babula, P.; Krska, B.; Horna, A.; Adam, V.; Provaznik, I.; Kizek, R. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against plum pox virus. Molecules 2010, 15, 6285-6305. 89. Sochor, J.; Skutkova, H.; Babula, P.; Zitka, O.; Cernei, N.; Rop, O.; Krska, B.; Adam, V.; Provaznik, I.; Kizek, R. Mathematical evaluation of content of amino acids and polyphenols and antioxidant activities of fruits from apricot cultivars. Molecules 2011, 16, 7428-7457. 90. Sochor, J.; Ryvolova, M.; Krystofova, O.; Salas, P.; Hubalek, J.; Adam, V.; Trnkova, L.; Havel, L.; Beklova, M.; Zehnalek, J.; et al. Fully automated spectrometric protocols for determination of an antioxidant activity: Advantages and disadvantages. Molecules 2010, 15, 8618-8640. 91. Rop, O.; Sochor, J.; Jurikova, T.; Zitka, O.; Skutkova, H.; Mlcek, J.; Salas, P.; Krska, B.; Babula, P.; Adam, V.; et al. Effect of five different stages of ripening on chemical compounds in medlar (mespilus germanica l.). Molecules 2011, 16, 74-91.
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