A sensor for investigating the interaction between biologically important heavy metals and glutathione

Húska, Dalibor; Zítka, Ondřej; Adam, Vojtěch; Beklová, Miroslava; Křížková, Soňa; Zeman, Ladislav; Horna, Aleš; Havel, Ladislav; Zehnálek, Josef; Kizek, René

dc.title	A sensor for investigating the interaction between biologically important heavy metals and glutathione	en
dc.contributor.author	Húska, Dalibor
dc.contributor.author	Zítka, Ondřej
dc.contributor.author	Adam, Vojtěch
dc.contributor.author	Beklová, Miroslava
dc.contributor.author	Křížková, Soňa
dc.contributor.author	Zeman, Ladislav
dc.contributor.author	Horna, Aleš
dc.contributor.author	Havel, Ladislav
dc.contributor.author	Zehnálek, Josef
dc.contributor.author	Kizek, René
dc.relation.ispartof	Czech Journal of Animal Science
dc.identifier.issn	1212-1819 Scopus Sources, Sherpa/RoMEO, JCR
dc.date.issued	2007-02
utb.relation.volume	52
utb.relation.issue	2
dc.citation.spage	37
dc.citation.epage	43
dc.type	article
dc.language.iso	en
dc.publisher	National Institute of Food and Agriculture (NIFA)	en
dc.identifier.doi	10.17221/2345-CJAS
dc.relation.uri	https://www.agriculturejournals.cz/web/cjas.htm?volume=52&firstPage=37&type=publishedArticle
dc.subject	reduced glutathione (GSH)	en
dc.subject	oxidized glutathione (GSSG)	en
dc.subject	thiols	en
dc.subject	cadmium	en
dc.subject	zinc	en
dc.subject	copper	en
dc.subject	nickel	en
dc.subject	additive	en
dc.subject	cyclic voltammetry	en
dc.subject	electrochemical method	en
dc.description.abstract	Glutathione (GSH) is a water-soluble tripeptide playing a crucial role in the number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in an oxidized (GSSG) and reduced (GSH) state. The aim of this paper was to use electrochemical methods to determine glutathiones and to investigate interactions of GSH and cadmium, zinc, copper and nickel ions or feed additive for animal nutrition. It clearly follows from the results that GSH and GSSG can be distinguished by cyclic voltammetry whereas the level of their current response is proportional to their concentration. Moreover, we studied these interactions by cyclic voltammetry. Marked changes in the GSH signal were observed and discussed. The highest decrease in a GSH reductive signal is caused by cadmium ions followed by zinc, nickel and copper ones. We also observed that the reductive GSH signal gradually decreased (about 9%) with the increasing concentration of feed additive used in our experiments. In conclusion, cyclic voltammetry offers a tool for studying interactions of thiols with different substances such as heavy metals and/or feed additives.	en
utb.faculty	Faculty of Technology
dc.identifier.uri	http://hdl.handle.net/10563/1002091
utb.identifier.obdid	43865415
utb.identifier.scopus	2-s2.0-33847327905
utb.identifier.wok	000244285800002
utb.source	j-wok
dc.date.accessioned	2011-08-16T15:06:27Z
dc.date.available	2011-08-16T15:06:27Z
utb.contributor.internalauthor	Horna, Aleš
utb.fulltext.affiliation	D. Húska1,2, O. Zítka2,3, V. Adam2,4, M. Beklová5, S. Křížková2,4, L. Zeman6, A. Horna7, L. Havel1, J. Zehnálek2, R. Kizek2 1 Department of Plant Biology, Mendel University of Agriculture and Forestry, Brno, Czech Republic 2 Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno, Czech Republic 6 Department of Animal Nutrition, Mendel University of Agriculture and Forestry, Brno, Czech Republic 3 Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic 4 Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic 5 Department of Veterinary Ecology and Environment Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 7 Department of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, Zlín, Czech Republic Corresponding Author Doc. Ing. René Kizek, PhD., Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, 613 00 Brno, Czech Republic Tel. +420 545 133 350, fax +420 545 212 044, e-mail: [email protected]
utb.fulltext.dates	Received: 2006–09–06 Accepted after corrections: 2006–12–07
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utb.fulltext.sponsorship	Supported by the Ministry of Education, Youth and Sports of the Czech Republic (Project No. MSMT 6215712402) and Czech Science Foundation (Grant No. 525/04/P132).
utb.fulltext.projects	MSM 6215712402
utb.fulltext.projects	GACR 525/04/P132