Selected cultivars of cornelian cherry (Cornus mas L.) as a new food source for human nutrition

Abstract

Cílem práce bylo stanovit antioxidační kapacitu u 12 vybraných odrůd dřínu obecného (Cornus mas L.). Byly použity 2 metody, a to ABTS a DPPH. Celkové fonoly byly stanoveny pomocí Folin-Ciocelteau činidla a pro určení vitaminu C bylo použito kapalinové chromatografie ? elektrochemický detektor (Coulochem III). Nejvyšší množství fenolů bylo u odrůdy ´Vydubeckij´ a ´Titus´, a to 7,96 a 8,11 g kyseliny galové.kg-1 čerstvé hmoty. Mezi antioxidační kapacitou a fenoly byly zjištěny vysoké hodnoty korelace (r2 = 0,970 pro DPPH test a r2 = 0,978 pro ABTS test). Nejvyšší obsah vitaminu C byl u odrůdy ´Olomoucky´, a to 3.11 g.kg-1 čerstvé hmoty.

The aim of this work was to determine antioxidant activity in 12 cultivars of cornelian cherry (Cornus mas L.). Two assays based on ion reduction of ABTS (2,2´-azinobis(3-ethylbenzothiazoline-6-sulphonate) and DPPH (1,1-Diphenyl-2-picrylhydrazyl) were used for antioxidant activity. Total phenolic content of the fruit was analysed by Folin-Ciocelteau colorimetric method and ascorbic acid content was analysed using column chromatography - electrochemical detector (Coulochem III). The highest amounts of total phenolic content were found in cvs. ´Vydubeckij´ and ´Titus´ as 7.96 and 8.11 g gallic acid.kg-1 of fresh mass (FM). High correlation between polyphenols and antioxidant activity in fruits of the cultivars was observed (r2 = 0.970 for DPPH test, and r2 = 0.978 for ABTS test). The highest total content of ascorbic acid was determined in cultivar ´Olomoucky´, with the value of 3.11 g.kg-1 FM. This study attempts to contribute to the knowledge of human nutritional properties of these cornelian cherry cultivars, and may be useful for the evaluation of dietary information and further propagation of cultivation and utilization of this fruit in the world.